Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lipopolysaccharide dose response in baboons.

Sandra B Haudek1, Beate E Natmessnig, Walter Fürst

  • 1Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, Austria.

Shock (Augusta, Ga.)
|October 16, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intraoperative Zoledronic Acid for Arthroscopic Rotator Cuff Repair Results in Distinct Circulating Micro-RNA Profiles Indicating Improved Tendon-to-Bone Healing.

The American journal of sports medicine·2026
Same author

Navigating the transition from basic science researcher to medical educator.

Advances in physiology education·2026
Same author

Transcription-independent induction of rapid-onset senescence is integral to healing.

Nature cell biology·2026
Same author

Interaction of pulsed low frequency electromagnetic field (PEMF) with mitochondria.

Scientific reports·2026
Same author

Intraoperative Zoledronic Acid for Arthroscopic Rotator Cuff Repair: Short-Term Results From a Prospective, Randomized, Placebo-Controlled Phase II Trial.

The American journal of sports medicine·2026
Same author

Femtosecond Laser Engraving Promotes the Repopulation of Decellularized Human Articular Cartilage.

Journal of tissue engineering and regenerative medicine·2026

Baboon endotoxemia models using lipopolysaccharide (LPS) show similar cytokine, hemodynamic, and hematologic changes as humans. This validates the baboon model for studying human sepsis pathogenesis.

Area of Science:

  • * Infectious Diseases
  • * Animal Models
  • * Immunology

Background:

  • * Lipopolysaccharide (LPS) from Escherichia coli is a potent endotoxin that triggers inflammatory responses.
  • * Understanding endotoxemia in humans is crucial for sepsis research.
  • * Animal models are essential for studying complex biological processes like sepsis.

Purpose of the Study:

  • * To establish and validate an experimental baboon model of endotoxemia.
  • * To compare LPS-induced responses in baboons with human endotoxemia data.
  • * To assess the utility of the baboon model for human sepsis research.

Main Methods:

  • * Acute and subacute endotoxemia models were induced in baboons using varying doses and infusion rates of LPS.
  • * Key parameters analyzed included LPS clearance, cytokine (TNF, IL-6, IL-8) expression at protein and mRNA levels, blood cell counts, and hemodynamic variables (temperature, cardiac index, heart rate, mean arterial pressure).

Related Experiment Videos

  • * Data collected from baboons were compared with existing human endotoxemia data.

    • Main Results:

    • * Baboons exhibited similar kinetics of cytokine release, hemodynamic, and hematologic changes compared to humans following LPS administration.
    • * A significantly higher initial LPS dose (approximately 104-fold) was required in baboons to elicit comparable responses.
    • * LPS clearance rates and expression patterns of inflammatory markers showed qualitative similarities between species.

    Conclusions:

    • * The baboon endotoxemia model qualitatively, but not quantitatively, mimics human endotoxemia.
    • * This model serves as a valuable tool for investigating the pathogenic mechanisms of sepsis in a human-relevant context.
    • * Further research using this validated baboon model can advance our understanding and treatment of sepsis.