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Human colon carcinomas constitutively express and shed type II IL-1 receptor, an IL-1 antagonist

Andrew W Stadnyk1, Moorix M W Yeung, Sen Rong Yan

  • 1Department of Pediatrics, Dalhousie Inflammation Group, Dalhousie University, Halifax, Nova Scotia, Canada.

Digestive Diseases and Sciences
|October 17, 2003
PubMed

Insights

Human colon cells express the IL-1 receptor type II (IL-1RII), an IL-1 antagonist. This finding suggests IL-1RII plays a role in regulating inflammation in the human gut.

Area of Science:

  • Immunology
  • Gastroenterology
  • Cell Biology

Background:

  • Rat intestinal cells express IL-1RII in response to helminth infection, NSAID injury, and detachment.
  • The role of IL-1RII in human intestinal epithelial cells remains largely unexplored.

Purpose of the Study:

  • To determine if human colon carcinoma cell lines express IL-1RII.
  • To investigate if IL-1RII expression can be induced in these cells.
  • To assess the functional significance of IL-1RII in human intestinal epithelial cells.

Main Methods:

  • Reverse transcription-polymerase chain reaction (RT-PCR) to detect IL-1RII mRNA.
  • Immunohistochemistry to detect IL-1RII protein.
  • Western blotting to estimate protein size.
  • Tumor Necrosis Factor (TNF) treatment to assess induction of IL-1RII.
  • Treatment with inhibitory anti-IL-1RII antibodies to measure IL-8 production.

Main Results:

  • T84 and HT-29 cell lines constitutively expressed mRNA for the membrane-bound IL-1RII.
  • TNF treatment increased IL-1RII mRNA and soluble protein levels in T84 cells.
  • Blocking IL-1RII function enhanced IL-1-induced IL-8 production in T84 cells.

Conclusions:

  • Human colon carcinoma cell lines express the membrane-bound IL-1RII.
  • IL-1RII expression can be modulated by TNF treatment.
  • IL-1RII acts as an antagonist to IL-1, influencing IL-8 production in human intestinal epithelial cells, with implications for inflammatory gut diseases.

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