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Aqueous two-phase systems for biomolecule separation.

A D Diamond1, J T Hsu

  • 1Department of Chemical Engineering, Lehigh University, Bethlehem, PA 18015.

Advances in Biochemical Engineering/Biotechnology
|January 1, 1992
PubMed
Summary
This summary is machine-generated.

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Aqueous polymer two-phase systems offer advanced biomolecule purification and bioconversion in biotechnology. This technology enables efficient separation and production of valuable biomolecules.

Area of Science:

  • Biotechnology
  • Separation Science

Background:

  • Aqueous polymer two-phase systems have matured over 30 years.
  • They are established for biomolecule purification and bioconversion.

Purpose of the Study:

  • To summarize developments in aqueous two-phase systems for biotechnology.
  • To cover phase diagrams, biomolecule partitioning, purification, and bioconversion.

Main Methods:

  • Discussing thermodynamics of phase formation.
  • Analyzing factors influencing biomolecule partitioning.
  • Reviewing large-scale purification and extractive bioconversion techniques.

Main Results:

  • Phase diagrams are influenced by polymer properties, temperature, and salt.

Related Experiment Videos

  • Biomolecule partitioning depends on system composition and derivatives.
  • Applications include large-scale protein extraction and continuous chromatography.

    • Conclusions:

    • Aqueous two-phase technology is versatile for biomolecule separation.
    • Extractive bioconversion offers simultaneous production and purification.