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Crystallization and preliminary X-ray analysis of class II fructose-1,6-bisphosphate aldolase from Thermus
Jun Hyuck Lee1, Young Jun Im, Seong-Hwan Rho
1Department of Life Science, Kwangju Institute of Science & Technology, Gwangju 500-712, Korea.
Abstract:
In this study, we have crystallized class II fructose-1,6-bisphosphate aldolase (FBA) from Thermus caldophilus (Tca). Purified Tca FBA is a tetrameric enzyme of 305 residues, which crystallizes in the space group P2(1)2(1)2(1) (cell dimensions a = 98.9, b = 113.1, c = 115.7 A), with four molecules in the asymmetric unit. A complete diffraction data set was obtained from orthorhombic crystals at resolution of 2.2 A.
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