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Related Experiment Videos

Cdk2 knockout mice are viable.

Cyril Berthet1, Eiman Aleem, Vincenzo Coppola

  • 1Regulation of Cell Growth Laboratory, National Cancer Institute, Building 560, 1050 Boyles St., Frederick, MD 21702-1201, USA.

Current Biology : CB
|October 17, 2003
PubMed
Summary
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Cyclin-dependent kinase 2 (Cdk2) is not essential for mouse survival but is crucial for germ cell development and meiosis. Loss of Cdk2 delays cell cycle progression into S phase.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Cyclin-dependent kinases (Cdks) and cyclins regulate cell cycle progression.
  • Cdk2/cyclin E complexes are critical for the G1/S transition.
  • Cdk2 also associates with cyclin A, important for embryonic proliferation.

Purpose of the Study:

  • To investigate the in vivo functions of Cdk2.
  • To determine the necessity of Cdk2 for mouse viability and development.

Main Methods:

  • Generation of Cdk2 knockout mice.
  • Analysis of kinase activity in Cdk2(-/-) extracts.
  • Assessment of cell proliferation and cell cycle progression in Cdk2(-/-) cells.

Main Results:

Related Experiment Videos

  • Cdk2 knockout mice are viable and fertile, indicating Cdk2 is not essential.
  • Cdk2 is required for germ cell development, leading to sterility in knockout mice.
  • Loss of Cdk2 causes delayed entry into S phase in mouse embryonic fibroblasts.

Conclusions:

  • Cdk2 is essential for germ cell development and meiosis, but not for overall mouse viability.
  • Cdk2 plays a role in regulating the timing of S phase entry in the mitotic cell cycle.