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Related Experiment Videos

Correlation between olfactory receptor cell type and function in the channel catfish.

Anne Hansen1, Shane H Rolen, Karl Anderson

  • 1Department of Cellular and Structural Biology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|October 17, 2003
PubMed
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Fish olfactory receptor neurons (ORNs) use distinct G-protein pathways to detect different odorants. Ciliated ORNs detect bile salts via Galpha(olf)/cAMP, while microvillous ORNs detect nucleotides and amino acids via different signaling cascades.

Area of Science:

  • Olfactory neuroscience
  • Fish sensory biology
  • Molecular signaling

Background:

  • Fish olfactory epithelium contains ciliated, microvillous, and crypt olfactory receptor neurons (ORNs).
  • Different ORN types may detect distinct odorant classes using specific receptor molecules and G-proteins.
  • Understanding ORN function is crucial for deciphering fish chemosensation.

Purpose of the Study:

  • To investigate whether different fish ORN types respond to specific odorant classes via distinct G-protein signaling pathways.
  • To correlate ORN morphology with their molecular and anatomical projections in the olfactory bulb.
  • To elucidate the transduction mechanisms underlying odorant detection in catfish.

Main Methods:

  • Retrograde tracing to map ORN projections in the olfactory bulb.

Related Experiment Videos

  • Pharmacological manipulation of G-protein signaling pathways (Galpha(olf)/cAMP and Galpha(q)/11/PLC).
  • Analysis of odorant-evoked responses in relation to ORN type and signaling pathway inhibitors.

    • Main Results:

    • Ciliated ORNs express OR-type receptors and Galpha(olf), projecting to medial olfactory bulb regions responding to bile salts.
    • Microvillous ORNs express Galpha(q)/11 or other G-proteins, projecting to dorsal olfactory bulb regions responding to nucleotides and amino acids.
    • Forskolin (inhibiting Galpha(olf)/cAMP) blocked bile salt responses and reduced amino acid responses; U-73122/U-73343 (inhibiting Galpha(q)/11/PLC) reduced amino acid responses but not bile salt or nucleotide responses.

    Conclusions:

    • Bile salts are primarily detected by ciliated ORNs via the Galpha(olf)/cAMP pathway.
    • Nucleotides are detected by microvillous ORNs using signaling cascades independent of Galpha(olf)/cAMP and Galpha(q)/11/PLC.
    • Amino acids activate both ciliated and microvillous ORNs, but through distinct transduction pathways in each cell type.