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Updated: Aug 30, 2026
Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro
Published on: August 14, 2011
Interactions between the membranes of turkey cells and Mycoplasma meleagridis
K M Lam1, A J DaMassa, G Yan Ghazikhanian
1Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Abstract:
We used Myoplsma meleagridis (MM) to infect the RP-9 cells and the eggshell membranes and scanning electron microscopy (SEM) and confocal microscopy to study the interactions between the organisms and the cell surfaces. The surface of the RP-9 cells contained numerous projections. After 24 hr of infection with MM, those projections were either lost or aggregated to the side; MM-like particles could be seen on the surface of the cells, and surface fluorescence could be detected by confocal microscopy. On the surface of MM-infected shell membranes were necrotic fibrous tissues and cells detected by SEM and an intense surface fluorescence detected by confocal microscopy. These results indicate that MM infection of the cell surface can result in cellular damage.
Insights
Mycoplasma meleagridis (MM) infection damages RP-9 cells and eggshell membranes. Researchers observed cellular changes and surface fluorescence, indicating significant harm from MM.
Area of Science:
- Cell Biology
- Microbiology
- Pathology
Background:
- Mycoplasma meleagridis (MM) is a pathogen known to affect avian species.
- Understanding the interaction of MM with host cells is crucial for disease management.
Purpose of the Study:
- To investigate the effects of Mycoplasma meleagridis (MM) infection on RP-9 cells and eggshell membranes.
- To characterize the interaction between MM and cell surfaces using advanced microscopy techniques.
Main Methods:
- Infection of RP-9 cells and eggshell membranes with Mycoplasma meleagridis (MM).
- Utilized scanning electron microscopy (SEM) to visualize surface morphology.
- Employed confocal microscopy to detect surface fluorescence and cellular changes.
Main Results:
- RP-9 cell surface projections were lost or aggregated after 24 hr of MM infection.
- MM-like particles and surface fluorescence were observed on infected cells.
- SEM revealed necrotic fibrous tissues and cells on infected eggshell membranes, with intense fluorescence detected by confocal microscopy.
Conclusions:
- Mycoplasma meleagridis (MM) infection causes significant cellular damage to RP-9 cells and eggshell membranes.
- MM interacts with cell surfaces, leading to observable pathological changes and fluorescence.
- Microscopic analysis provides insights into the cytopathic effects of MM.
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