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Related Experiment Videos

Functional endothelial cells derived from rhesus monkey embryonic stem cells.

Dan S Kaufman1, Rachel L Lewis, Eric T Hanson

  • 1Wisconsin Priamte Research Center, Laboratory of Developmental Biology and Department of Anatamy, University of Wisconsin, Madison, USA. kaufm020@umn.edu

Blood
|October 18, 2003
PubMed
Summary

Rhesus monkey embryonic stem cells successfully differentiated into endothelial cells (RESDECs). These RESDECs formed capillary-like structures and blood vessels in vivo, promoting tumor vascularization and offering new therapeutic strategies.

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Area of Science:

  • Stem Cell Biology
  • Developmental Biology
  • Vascular Biology

Background:

  • Endothelial cells are crucial for blood vessel formation.
  • Studying endothelial cell development requires suitable cell models.
  • Primate embryonic stem cells offer a potential model for human development.

Purpose of the Study:

  • To investigate the potential of rhesus monkey embryonic stem cells (ESCs) for endothelial cell differentiation.
  • To characterize the properties and in vivo functionality of these derived endothelial cells.
  • To explore their utility in studying vascular development and potential therapeutic applications.

Main Methods:

  • Rhesus ES cells (R366.4) were cultured with growth factors (VEGF, bFGF, IGF, EGF).
  • Differentiated cells (RESDECs) were analyzed for morphology, phenotype (CD146, vWF, integrin αvβ3, UEA-1), and LDL uptake.

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  • In vitro capillary-like structure formation in Matrigel was assessed.
  • In vivo studies involved Matrigel plug assays and co-injection with tumor cells in mice.

    • Main Results:

    • Rhesus ES cells differentiated into cells with endothelial morphology and phenotype (RESDECs).
    • RESDECs formed capillary-like structures in vitro and functional blood vessels in vivo.
    • Co-injection of RESDECs with tumor cells enhanced tumor growth and vascularization.
    • RESDECs expressed key endothelial markers and receptors (Flk-1) and secreted VEGF.

    Conclusions:

    • Rhesus ES cells can be reliably differentiated into functional endothelial cells (RESDECs).
    • RESDECs serve as a valuable model for studying endothelial cell development and angiogenesis.
    • This model holds promise for developing novel therapeutic strategies targeting vascularization.