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Updated: Aug 4, 2026
Creation of Reversible Cholestatic Rat Model
Published on: May 21, 2011
Bacteria ascend to liver from the bilioenteric conduit after choledochojejunostomy in the cholestatic rat
C S Hsieh1, L-T Huang, C C Huang
1Department of Pediatric Surgery, Chang Gung Memorial Hospital, Kaohsiung and the Chang Gung University, 123 Ta-Pei Road, Niao-Sung Hsiang, Kaohsiung Hsien, Taiwan. swjjymcs@ms17.hinet.net
Insights
Bacteria can ascend to the liver after Roux-Y choledochojejunostomy (RYCJ), especially in jaundiced rats. This study suggests a model for ascending cholangitis, crucial for understanding post-surgical infections.
Area of Science:
- Hepatobiliary Surgery
- Surgical Infections
- Microbiology
Background:
- Postoperative cholangitis is common after Roux-Y choledochojejunostomy (RYCJ), particularly in children.
- Jaundiced patients are more susceptible to bile duct infections following bilioenteric anastomosis.
Purpose of the Study:
- To investigate bacterial ascent to the liver following RYCJ in an animal model.
- To compare bacterial presence in jaundiced versus non-jaundiced rats after experimental inoculation.
Main Methods:
- Rats underwent RYCJ, with some subjected to prior bile duct ligation to induce jaundice.
- Escherichia coli or sterile saline was injected into the bilioenteric conduit (BEC).
- Quantitative bacterial cultures and pulse-field gel electrophoresis (PFGE) were used to analyze samples from the choledochojejunostomy and liver.
Main Results:
- A significant increase in E. coli was detected in the liver of jaundiced rats that received the bacterial injection.
- Bacterial presence in the liver was significantly higher in jaundiced rats compared to non-jaundiced controls.
- PFGE confirmed the injected E. coli strain in the liver samples.
Conclusions:
- Bacteria can ascend early to the liver from the BEC after RYCJ.
- Jaundice exacerbates bacterial ascent and infection risk in the post-RYCJ setting.
- This study establishes a potential animal model for further research into ascending cholangitis.
Abstract:
The high incidence of postoperative cholangitis in children with clinical restoration of bile flow after Roux-Y choledochojejunostomy (RYCJ) assumed the concept of a direct ascending cholangitis caused by pathogens in the intestine, into the intrahepatic bile duct via the porta hepatis. It is also well known that jaundiced animals (patients) are more susceptible to infections of the bile ducts following the procedure of bilioenteric anastomosis. An animal experiment was conducted to compare quantitative bacterial cultures of the choledochojejunostomy area and the liver 24 hours after Escherichia coli (ATCC 25922) or sterile normal saline was injected into the bilioenteric conduit (BEC), following RYCJ in rats with or without the proceeding bile duct ligation. A significant increase of E. coli of the same strain (ATCC 25922), that we injected into the BEC, was proved with pulse-field gel electrophoresis (PFGE) and shown in the liver of the jaundiced rats receiving E. coli (ATCC 25922), compared to that in the nonjaundiced rats with normal saline treatment. It is concluded that bacteria often ascend early to the liver from the BEC following RYCJ. This ascending cholangitis model might be produced for further studies.
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