Accuracy and precision of computerized models of the anterior cranial base in young mice

The quantitative analysis of craniofacial growth in experimental animals relies on computerized reconstructions in order to measure changes in form. The purpose of this study was to determine the validity of computerized three-dimensional models of the anterior cranial base in mice. Ten 1-day-old non-littermates were collected and the anterior cranial base was dissected free from surrounding connective tissues. Eleven measurements were recorded from these cartilages. Twenty developmentally equivalent mice were collected and fixed with either glutaraldehyde or formalin and the anterior cranial base from each specimen was subjected to computerized reconstruction. The corresponding 11 measurements were recorded from these models. Results showed that the measurements recorded from the computerized models were not significantly different from those recorded directly from the actual anterior cranial bases. Therefore, the reconstructions were considered accurate. An analysis of the coefficients of error revealed that measurements derived from the computerized models were significantly more precise than those recorded directly from the actual tissue. The computerized three-dimensional reconstruction method provides accurate and precise models of the anterior cranial base in young mice.