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LNA and alpha-L-LNA: towards therapeutic applications
Jesper Wengel1, Birte Vester, Lars Bo Lundberg
1Department of Chemistry, Nucleic Acid Center, University of Southern Denmark, Odense M, Denmark. jwe@chem.sdu.dk
Nucleosides, Nucleotides & Nucleic Acids
|October 21, 2003
Abstract:
LNA and alpha-L-LNA are promising candidates for the development of efficient oligonucleotide-based therapeutic agents. Here, we report dose-dependent inhibition of HIV-1 Tat-dependent trans activation by a 12-mer chimeric alpha-L-LNA/DNA oligomer. This oligomer exhibits a dose-dependency similar to that of the corresponding 12-mer chimeric LNA/2'-O-Me-RNA oligomer. In addition, we show that incorporation of alpha-L-LNA or LNA monomers into each of the two binding arms of a "10-23" DNAzyme markedly increases cleavage of the target RNA.