Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Novel base-labile protecting groups for 5'-hydroxy function in solid-phase oligonucleotide synthesis.

Kazuo Kamaike1, Takashi Namiki, Etsuko Kawashima

  • 1School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan. kamaikek@ps.toyaku.ac.jp

Nucleosides, Nucleotides & Nucleic Acids
|October 21, 2003
PubMed
Summary

Novel base-labile protecting groups, 6-(levulinyloxymethyl)-3-methoxy-2-nitrobenzoyl (LMMoNBz) and 2-(levulinyloxymethyl)-5-methoxy-4-nitrobenzoyl (LMMpNBz), were developed for oligonucleotide synthesis. The LMMoNBz group demonstrated strong feasibility in comparative studies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spectral presaturation with inversion recovery provides superior neuromelanin imaging for Parkinson's disease evaluation compared to magnetization transfer.

Neuroradiology·2026
Same author

Coronary artery black-blood imaging via T<sub>2</sub>-prepared phase-sensitive inversion-recovery steady-state free precession in Kawasaki disease.

Magnetic resonance imaging·2025
Same author

Detour canal, a civil engineering heritage created through historical struggle for water resources, now provides the habitats for a rare freshwater fish.

Biodiversity data journal·2024
Same author

Comparison of 3D Magnetization-transfer- and Spectral-presaturation-with-inversion-recovery-based Neuromelanin Imaging.

Magnetic resonance in medical sciences : MRMS : an official journal of Japan Society of Magnetic Resonance in Medicine·2024
Same author

Total Synthesis of Marine Polyketide Plakortone Q.

Chemical & pharmaceutical bulletin·2024
Same author

Synthesis and Evaluation of MGB Polyamide-Oligonucleotide Conjugates as Gene Expression Control Compounds.

Journal of nucleic acids·2023

Area of Science:

  • Organic Chemistry
  • Biochemistry
  • Molecular Biology

Background:

  • Solid-phase oligonucleotide synthesis requires efficient protection of the 5'-hydroxy function.
  • Existing protecting groups may have limitations in stability or cleavage efficiency.
  • Development of novel protecting groups is crucial for advancing synthetic capabilities.

Purpose of the Study:

  • To develop and evaluate novel base-labile protecting groups for the 5'-hydroxy function in oligonucleotide synthesis.
  • To compare the performance of newly developed groups with existing ones.

Main Methods:

  • Synthesis of 6-(levulinyloxymethyl)-3-methoxy-2-nitrobenzoyl (LMMoNBz) and 2-(levulinyloxymethyl)-5-methoxy-4-nitrobenzoyl (LMMpNBz) protecting groups.
  • Incorporation of these groups into solid-phase oligonucleotide synthesis protocols.

Related Experiment Videos

  • Comparative analysis of protecting group efficiency and base-lability.

    • Main Results:

    • Successful development of LMMoNBz and LMMpNBz as novel protecting groups.
    • Demonstrated strong feasibility and effectiveness of the LMMoNBz group in oligonucleotide synthesis.
    • Comparative study highlighted the advantages of LMMoNBz over LMMpNBz and LMNBz (2-(levulinyloxymethyl)-5-nitrobenzoyl).

    Conclusions:

    • The LMMoNBz group is a promising novel protecting group for 5'-hydroxy function in solid-phase oligonucleotide synthesis.
    • This development offers an improved tool for efficient and reliable oligonucleotide synthesis.
    • Further studies may explore its application in complex oligonucleotide synthesis.