Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nucleobase modified peptide nucleic acid.

R H E Hudson1, R D Viirre, N McCourt

  • 1Department of Chemistry, The University of Western Ontario, London, Ontario, Canada. rhhudson@uwo.ca

Nucleosides, Nucleotides & Nucleic Acids
|October 21, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Shielding commissioning factors in nuclear medicine facilities.

Journal of radiological protection : official journal of the Society for Radiological Protection·2019
Same author

Position paper: recommendations for a digital mammography quality assurance program V4.0.

Australasian physical & engineering sciences in medicine·2017
Same author

Novel characterization of bEnd.3 cells that express lymphatic vessel endothelial hyaluronan receptor-1.

Lymphology·2014
Same author

How accurate is the electronic health record? - a pilot study evaluating information accuracy in a primary care setting.

Studies in health technology and informatics·2011
Same author

Fetoscopy under gas amniodistension: pressure-dependent influence of helium vs nitrous oxide on fetal goats.

Journal of pediatric surgery·2007
Same author

Doctors practising alternative medicine--the legal considerations.

Hong Kong medical journal = Xianggang yi xue za zhi·2006

This study introduces a palladium/copper-catalyzed cross-coupling method to modify peptide nucleic acid (PNA) monomers with alkynes. This technique yields base-modified PNA oligomers, with potential for creating fluorescent derivatives.

Area of Science:

  • Organic Chemistry
  • Medicinal Chemistry
  • Biochemistry

Background:

  • Peptide nucleic acids (PNAs) are DNA/RNA mimics with therapeutic potential.
  • Modifying PNA bases is crucial for expanding their functionality.
  • Developing efficient synthetic routes for modified PNAs is an active research area.

Purpose of the Study:

  • To establish a Pd/Cu catalyzed cross-coupling method for introducing alkyne modifications onto PNA monomers.
  • To synthesize novel base-modified PNA oligomers.
  • To explore the potential for generating fluorescent PNA derivatives.

Main Methods:

  • Utilized palladium (Pd0) and copper (Cu1) catalysis for cross-coupling reactions.
  • Employed terminal alkynes as coupling partners.

Related Experiment Videos

  • Reacted iodinated nucleobases (5-iodouracil and 5-iodocytosine) on PNA monomers/submonomers.
  • Investigated reaction conditions to control product formation.

    • Main Results:

    • Successfully coupled terminal alkynes to iodinated PNA monomers and submonomers.
    • Achieved moderate to good yields for the cross-coupling reaction.
    • Observed subsequent ring-closing reactions under specific conditions, yielding fluorescent furano- and pyrrolo-fused PNA derivatives.

    Conclusions:

    • The Pd/Cu catalyzed cross-coupling is an effective strategy for synthesizing base-modified PNA oligomers.
    • The reaction allows for the introduction of diverse modifications via terminal alkynes.
    • The method provides a route to novel fluorescent PNA analogues with potential applications in diagnostics and therapeutics.