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Updated: Aug 3, 2026

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
4-Guanidino-2-pyrimidinone nucleobases: synthesis and hybridization properties
J Robles1, A Grandas, E Pedroso
1Departament de Química Orgànica, Facultat de Química, Universitat de Barcelona, Barcelona, Spain. jrobles@qo.ub.es
Abstract:
N-Alkylated 4-guanidino-2-pyrimidinone-containing nucleosides, in which the guanidine group mimics the double hydrogen bond donor pattern of protonated cytosine, were introduced in polypyrimidine sequences to explore their triple-helix forming capabilites. UV and CD melting experiments showed that strands containing these base analogues did not form triplex complexes.
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