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Synthesis and triplex binding properties of oligonucleotides containing a novel nucleobase
Florence Lecubin1, Michel Devys, Jean Louis Fourrey
1Institut de Chimie des Substances Naturelles, CNRS, 91198, Gif sur Yvette, France.
Nucleosides, Nucleotides & Nucleic Acids
|October 21, 2003
Abstract:
The thiazolo-indole compound 1 bearing the complementary donor-acceptor-donor sites (dad) was designed for specific recognition of an AT inverted base pair in pyrimidine triple helix motif. It was successfully incorporated into 14-mer oligonucleotide using a serinol unit as sugar derivative. The triple helix hybridization studies were examined by means of thermal denaturation experiments with a 26-mer DNA duplex containing the AT inverted base pair.