A novel and convenient method for the synthesis of free 5'-thiol modified oligonucleotides
1Nucleic Acids Laboratory, Department of Medicinal Chemistry, University of Szeged, Szeged, Hungary. kupi@ovrisc.mdche.u-szeged.hu
Nucleosides, Nucleotides & Nucleic Acids
|October 21, 2003
Abstract:
The synthesis of free 5'-thiol-modified oligonucleotides using a 4,4',4''-trimethoxytrityl (TMTr)-protected linker and standard Poly-Pak purification has been described.
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Strong bases like NaOH or KOH deprotonate the phthalimide to form the corresponding anion, which acts as a nucleophile. Further, the anion attacks an...
Strong bases like NaOH or KOH deprotonate the phthalimide to form the corresponding anion, which acts as a nucleophile. Further, the anion attacks an...