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New reagent for protein-DNA contacts footprinting
O A Koval1, S B Oleinikova, E L Chernolovskaya
1Laboratory of Nucleic Acids Biochemistry, Institute of Bioorganic Chemistry, Novosibirsk, Russia. o.koval@niboch.nsc.ru
Nucleosides, Nucleotides & Nucleic Acids
|October 21, 2003
Abstract:
We have found, that the reaction of o-bromobenzoic acid with Cu2+ ions can be used as a source of activated oxygen species capable of cleaving DNA. Possibility to apply this reaction for footprinting the nucleosome core in the reconstituted chromatin was demonstrated.