Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endcaps for stabilizing short DNA duplexes.

Pei-Sze Ng1, Maneesh R Pingle, Ganesan Balasundarum

  • 1Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907-1333, USA.

Nucleosides, Nucleotides & Nucleic Acids
|October 21, 2003
PubMed
Summary
This summary is machine-generated.

New DNA endcaps were synthesized to link hydroxyl groups in double-stranded DNA. These endcaps stabilize DNA duplexes, as confirmed by thermal melting experiments on modified DNA strands.

Related Experiment Videos

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatial Immune Model of Alveolar Lung Infection (SIMALI) Identifies Structural Determinants of Lung Inflammation.

Research squareยท2026
Same author

Quantitative live-imaging platform to probe the spatio-temporal internalization and lysosomal degradation of therapeutic antibodies and ADCs in cells.

Journal of immunoassay & immunochemistryยท2026
Same author

Cytotoxic and oxidative effects of commercially available propylene glycol (PG) and vegetable glycerin (VG): Common humectants in electronic cigarettes.

Toxicology reportsยท2025
Same author

Modulation of corticospinal excitability by paired associative stimulation following stroke.

Clinical neurophysiology : official journal of the International Federation of Clinical Neurophysiologyยท2025
Same author

Domains for inclusion in a novel Treatment Response Measure for Systemic Lupus Erythematosus (TRM-SLE): results of a modified Delphi study.

Lupus science & medicineยท2025
Same author

Rehabilitation under fire: insights from SUMC.

Lancet (London, England)ยท2025
Same journal

LncRNA EGOT inhibits the proliferation of lung adenocarcinoma cells and is associated with poor prognosis.

Nucleosides, nucleotides & nucleic acidsยท2026
Same journal

Can the combination of 5-fluorouracil and gemcitabine increase the treatment efficacy of pancreatic and colon cancer?

Nucleosides, nucleotides & nucleic acidsยท2026
Same journal

"Aptamer-guided therapeutics and diagnostics for metabolic syndrome: Design, delivery, and translation of aptamer-based systems".

Nucleosides, nucleotides & nucleic acidsยท2026
Same journal

The impact of vitamin B12 deficiency on urinary profile of <i>de novo</i> purine synthesis intermediates: consequences of the methylfolate trap.

Nucleosides, nucleotides & nucleic acidsยท2026
Same journal

Evaluation of MTH1 and DCTPP1 inhibitors in fluorocyclopentenylcytosine (RX-3117) resistant lung cancer cells and potential cross resistance patterns.

Nucleosides, nucleotides & nucleic acidsยท2026
Same journal

Circular RNAs: promising biomarkers for diagnosis, prognosis and therapy in oral squamous cell carcinoma.

Nucleosides, nucleotides & nucleic acidsยท2026
See all related articles

Area of Science:

  • Chemical Synthesis
  • Molecular Biology
  • Biochemistry

Background:

  • Double-stranded DNA (dsDNA) stability is crucial for molecular biology applications.
  • Covalent modification of DNA ends can enhance duplex stability.
  • Existing methods for DNA end modification are limited.

Purpose of the Study:

  • To synthesize novel endcaps for covalent linkage of 3' and 5' hydroxyl groups in blunt-ended dsDNA.
  • To evaluate the stabilizing effect of these endcaps on DNA duplexes.

Main Methods:

  • Synthesis of endcap diols.
  • Conversion of endcap diols to DMTr-protected phosphoramidites.
  • Incorporation of phosphoramidites into a self-complementary octamer sequence.
  • Determination of duplex DNA stability using Tm (melting temperature) experiments.

Main Results:

  • Successful synthesis of endcap phosphoramidites.
  • Demonstrated incorporation of endcaps into DNA strands.
  • Tm experiments showed a stabilizing effect of the endcaps on the DNA duplex.

Conclusions:

  • The developed endcaps effectively stabilize blunt-ended double-stranded DNA.
  • This method offers a new strategy for enhancing DNA structural integrity.
  • The endcaps have potential applications in DNA-based technologies.