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Updated: Aug 8, 2026
Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
Published on: June 5, 2015
Development of an animal-human antibody complex for use as a control in ELISA
Mi Li Gu1, S L Feng, J K Glenn
1BioAnalytical Sciences Development Department, Human Genome Sciences Inc., Rockville, MD 20850, USA. mili_gu@hgsi.com
Abstract:
In order to provide the equivalent of a human anti-human protein antibody as positive control in ELISAs, a goat-human antibody complex was created using chemical cross-linking. The resulting hybrid complex had a larger molecular size on HPLC and SDS-PAGE. In ELISA, the goat-human complex bound to human antigen and was detectable by a secondary anti-human conjugate. The method to make the hybrid complex is simple, cost-effective and can be used to make human-like antibodies to many human proteins.
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