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CD3+ and CD4+ cells adoptively transfer experimental hypersensitivity pneumonitis
1Department of Medicine and Pathology, Albuquerque Veterans Administration Medical Center, NM 87108.
The American Review of Respiratory Disease
|December 1, 1992
Summary
CD4+ and CD3+ T cells are key for transferring experimental hypersensitivity pneumonitis (EHP) in mice. Sensitized cells persist long-term, but their ability to transfer EHP decreases over time.
Area of Science:
- Immunology
- Pulmonology
- Cell Biology
Background:
- Experimental hypersensitivity pneumonitis (EHP) is an inflammatory lung disease.
- Identifying the specific immune cells responsible for EHP transfer is crucial for understanding its pathogenesis.
Purpose of the Study:
- To characterize the immune cell populations responsible for the adoptive transfer of murine EHP.
- To investigate the duration of sensitization and the ability of different cell populations to transfer EHP.
Main Methods:
- Spleen cell (SC) cultures from Micropolyspora faeni (M. faeni)-sensitized mice were depleted of CD3+, CD4+, or CD8+ cells.
- Adoptive transfer of these cells into recipients was assessed by measuring pulmonary inflammatory response after M. faeni challenge.
- Lung-associated lymph node (LALN) cells and SC from donors with varying challenge durations were also tested.
Main Results:
- Depletion of CD3+ and CD4+ cells, but not CD8+ cells, significantly reduced or abolished the capacity of SC to transfer EHP.
- Sensitized cells remained capable of transferring EHP for at least 8 weeks.
- Cultured LALN cells could transfer EHP, and SC from donors challenged for longer periods (4 and 8 weeks) resulted in less severe pulmonary abnormalities compared to 2-week donors.
Conclusions:
- The primary cells mediating adoptive transfer of EHP in this model are CD3+, CD4+, and CD8- T cells.
- While cell phenotype is critical, the duration of donor sensitization influences the efficacy of EHP transfer.