Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA helicase gene interaction network defined using synthetic lethality analyzed by microarray.

Siew Loon Ooi1, Daniel D Shoemaker, Jef D Boeke

  • 1Department of Molecular Biology & Genetics, The Johns Hopkins University School of Medicine, 617 Hunterian Building, 725 North Wolfe Street, Baltimore, Maryland 21205, USA.

Nature Genetics
|October 21, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genome-wide histone humanization in yeast disrupts genome organization, replication, and rDNA stability.

Cell reports·2026
Same author

Author Correction: Physiology and immunology of a pig-to-human decedent kidney xenotransplant.

Nature·2026
Same author

Improved vector toolkit for genome writing in mammalian cells.

bioRxiv : the preprint server for biology·2026
Same author

Retrotransposon Activation in the Aged and Alzheimer's Disease Brain Examined by Nanopore Long-read DNA Sequencing.

bioRxiv : the preprint server for biology·2026
Same author

To make biology programmable, we must master its generative grammar.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Ancient co-option of LTR retrotransposons as yeast centromeres.

Nature·2026
Same journal

Mutational scanning reveals substrate-assisted autoregulation of the WNT destruction complex.

Nature genetics·2026
Same journal

Spatial transcriptomic analyses highlight distinct erythroid niches in mice and humans.

Nature genetics·2026
Same journal

Building up pangenome analysis block by block.

Nature genetics·2026
Same journal

Mutations in splicing factor gene U2AF1 rescue defective oncogene splicing in KRAS-mutant cancers.

Nature genetics·2026
Same journal

Assessing the effect of immune surveillance on clonal expansions in the blood.

Nature genetics·2026
Same journal

Improved heritability partitioning and enrichment analyses using summary statistics with graphREML.

Nature genetics·2026
See all related articles

Synthetic lethality analysis by microarray (SLAM) efficiently identifies gene functions using DNA helicase mutants. This method reveals new genetic interactions and clarifies the roles of SGS1 and SRS2 in DNA replication and stress response.

Area of Science:

  • Genetics
  • Molecular Biology
  • Biochemistry

Background:

  • Understanding gene function and interactions is crucial in molecular biology.
  • DNA helicases play vital roles in DNA replication, repair, and recombination.
  • Synthetic lethality is a powerful approach to uncover functional relationships between genes.

Purpose of the Study:

  • To introduce and validate a new technique called synthetic lethality analysis by microarray (SLAM).
  • To identify redundant and unique biological functions of the DNA helicase genes SGS1 and SRS2.
  • To construct and analyze a DNA helicase genetic network.

Main Methods:

  • Utilized approximately 4,600 Saccharomyces cerevisiae haploid deletion mutants with molecular barcodes (TAGs).
  • Employed integrative transformation to introduce 'query mutations' (SGS1, SRS2) into the deletion pool, creating double mutants.

Related Experiment Videos

  • Optimized integrative transformation efficiency for successful SLAM implementation.

    • Main Results:

    • Defined a DNA helicase genetic network, revealing synthetic interactions.
    • Predicted distinct roles for SRS2 (replication fork processing) and SGS1 (rDNA replication, DNA topology, lagging strand synthesis).
    • Identified parallel pathway function of SGS1/SRS2 with MRC1 in DNA replication stress signaling and interaction with RRM3 in preventing recombination intermediates.

    Conclusions:

    • SLAM is an efficient method for detecting synthetic lethality and ranking genetic interactions.
    • The study elucidated specific roles for SGS1 and SRS2 in DNA replication and stress response pathways.
    • Established a framework for using SLAM to dissect complex genetic networks.