Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunofluorescence stimulated emission depletion microscopy.

Marcus Dyba1, Stefan Jakobs, Stefan W Hell

  • 1Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Göttingen, Germany.

Nature Biotechnology
|October 21, 2003
PubMed
Summary

This study demonstrates super-resolution microscopy using stimulated emission depletion, achieving 50 nm axial resolution. Standard immunofluorescence preparations are compatible with this advanced imaging technique for enhanced biological visualization.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Uninterrupted optical resolution of identical point scatterers undergoing nanometric changes in distance.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Addendum: In situ architecture of the human prohibitin complex.

Nature cell biology·2026
Same author

Phenoxazines with a Phototransferable <i>N</i>-Acetyl Group and Acrylate Linker: Assembly by C-H Activation, Photoconversion to Fluorescent Dyes, Biolabeling, and Super-Resolution Imaging.

Journal of the American Chemical Society·2026
Same author

A versatile nanobody platform for live and super-resolution imaging of synaptic vesicle dynamics and plasticity in rodent and human neurons.

Journal of nanobiotechnology·2026
Same author

Synthesis of Benzo[<i>b</i>]thiophene 1,1-Dioxides via Pd-Catalyzed Sulfinylation of Aryl Triflates and Their Use as Large Stokes Shift Fluorophores for Multicolor Live-Cell Imaging with Self-Labeling Tags.

JACS Au·2026
Same author

Photoactivatable Carborhodol and Carborhodamine Dyes with One Cleavable Group: Synthesis, Spectra, and Fluorescence Nanoscopy Applications.

JACS Au·2026

Area of Science:

  • Biophysics
  • Optical Microscopy
  • Immunofluorescence Imaging

Background:

  • Diffraction limits traditionally restrict the resolution of light microscopy.
  • Achieving nanoscale resolution is crucial for detailed cellular and molecular studies.

Purpose of the Study:

  • To report immunofluorescence imaging with spatial resolution beyond the diffraction limit.
  • To demonstrate the feasibility of subdiffraction resolution using standard immunofluorescence techniques.

Main Methods:

  • Utilized stimulated emission depletion (STED) microscopy with opposing lenses.
  • Achieved an axial resolution of approximately 50 nm (1/16 of the 793 nm irradiation wavelength).

Main Results:

  • Demonstrated subdiffraction resolution in immunofluorescence imaging.

Related Experiment Videos

  • Confirmed the stability of antibody-tagged labels under STED microscopy conditions.
  • Showcased the compatibility with standard immunofluorescence sample preparation.

    • Conclusions:

    • Stimulated emission depletion microscopy enables super-resolution imaging in standard immunofluorescence preparations.
    • This technique offers significantly improved axial resolution for biological samples.
    • Antibody-tagged labels are suitable for high-resolution imaging with STED microscopy.