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Related Experiment Videos

A new method using a bacterium for dissolution of urinary stones.

S Nishio1, K Aoki, M Yokoyama

  • 1Department of Urology, Ehime University School of Medicine, Ehime, Japan. nishio@m.ehime-u.ac.jp

Aktuelle Urologie
|October 21, 2003
PubMed
Summary

Thiobacillus thiooxidans effectively dissolves calcium phosphate urinary stones in rats. This bacterium shows potential for treating urinary stones, but further research is needed for clinical application.

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Area of Science:

  • Microbiology
  • Urology
  • Biotechnology

Background:

  • Thiobacillus thiooxidans utilizes inorganic sulfur compounds for energy, producing sulfuric acid.
  • Urinary stone dissolution is a significant clinical challenge.
  • Investigating bacterial dissolution of urinary stones offers a novel therapeutic approach.

Purpose of the Study:

  • To evaluate the efficacy of Thiobacillus thiooxidans in dissolving human urinary stones.
  • To determine optimal conditions for bacterial dissolution of urinary stones in human urine.
  • To assess the safety and feasibility of this method in an in vivo model.

Main Methods:

  • Cultured Thiobacillus thiooxidans and assessed its growth in human urine with varying sodium thiosulfite concentrations.
  • Examined urinary stone dissolution in Wistar rats inoculated with Thiobacillus thiooxidans and administered sodium thiosulfite.

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  • Monitored histological changes in urinary tracts and conducted blood tests for safety assessment.

    • Main Results:

    • Bacterial growth in human urine correlated with sodium thiosulfite concentration.
    • Calcium phosphate stones showed significant dissolution (78% reduction in dry weight).
    • Calcium oxalate, uric acid, and magnesium phosphate stones were less affected; control rats showed stone growth.

    Conclusions:

    • Thiobacillus thiooxidans demonstrates potential for dissolving urinary stones, particularly calcium phosphate types.
    • This bacterial-based approach may be beneficial for residual stone fragments post-ESWL or encrustations on urinary stents.
    • Further research is required to optimize conditions and establish safety protocols for clinical implementation.