Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

STOP proteins.

Christophe Bosc1, Annie Andrieux, Didier Job

  • 1Laboratoire du Cytosquelette, INSERM U366, DRDC/CS, CEA-Grenoble, 17 rue des Martyrs, F-38054 Grenoble Cedex 9, France. cbosc@cea.fr

Biochemistry
|October 22, 2003
PubMed
Summary

Microtubule-associated proteins called STOPs stabilize cellular microtubules against cold. STOP deficiency in mice causes synaptic defects, suggesting their role in neuroplasticity and potential use in studying schizophrenia.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ARMC2 loss impairs cilia structure and leads to primary ciliary dyskinesia symptoms in mouse organs.

Frontiers in cell and developmental biology·2026
Same author

Proper localization of the SCA13-linked K<sup>+</sup> channel in Purkinje neurons requires a microtubule-stabilizing protein.

Neurobiology of disease·2026
Same author

The Mn-motif protein MAP6d1 assembles ciliary doublet microtubules.

Nature communications·2025
Same author

Stable GDP-tubulin islands rescue dynamic microtubules.

The Journal of cell biology·2024
Same author

A cytoskeleton-membrane interaction conserved in fast-spiking neurons controls movement, emotion, and memory.

Molecular psychiatry·2023
Same author

VASH1-SVBP and VASH2-SVBP generate different detyrosination profiles on microtubules.

The Journal of cell biology·2022

Area of Science:

  • Cell Biology
  • Neuroscience
  • Protein Biochemistry

Background:

  • In vitro microtubules are cold-labile, but cellular microtubules resist cold.
  • Known microtubule-associated proteins (MAPs) like MAP2 and tau do not confer cold stability.
  • The mechanism behind cellular microtubule cold resistance remained unclear.

Purpose of the Study:

  • To investigate the role of STOPs (Stable Tubule Only Polypeptides) in microtubule cold stabilization.
  • To understand the structure-function relationship of STOP proteins.
  • To explore the in vivo function of STOPs in neuronal plasticity and behavior.

Main Methods:

  • In vitro microtubule assembly and depolymerization assays.
  • Biochemical analysis of calmodulin and microtubule binding domains of STOPs.
  • Generation and analysis of STOP-suppressed or deficient mouse models.

Main Results:

  • STOPs confer significant cold stabilization to microtubules in vitro.
  • STOPs possess distinct calmodulin- and microtubule-binding motifs.
  • STOP suppression in mice leads to synaptic defects and neuroleptic-sensitive behavioral alterations.
  • STOPs exhibit cell-specific variability due to alternative splicing and promoter usage.

Conclusions:

  • STOPs are crucial for maintaining microtubule stability, particularly against cold stress.
  • STOPs play a vital role in synaptic plasticity and neuronal function.
  • STOP-deficient mice provide a valuable model for studying neurodevelopmental and psychiatric disorders like schizophrenia.

Related Experiment Videos