Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

EWS/FLI function varies in different cellular backgrounds.

Jeffrey P Zwerner1, Jennifer Guimbellot, William A May

  • 1Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35294-1150, USA.

Experimental Cell Research
|October 22, 2003
PubMed
Summary
This summary is machine-generated.

Ewing sarcoma oncogene EWS/FLI

Related Experiment Videos

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Effects of Androgens on Scar Formation: A Systematic Review.

Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.]·2025
Same author

Single-cell immunopathology of recurrent acute generalized exanthematous pustulosis associated with vancomycin.

The journal of allergy and clinical immunology. Global·2025
Same author

Coagulation abnormalities following brown recluse spider (Loxosceles reclusa) envenomation: A description of 2 cases and review of the literature.

American journal of clinical pathology·2025
Same author

Extradigital Symplastic Glomus Tumor and Review of the Literature.

The American Journal of dermatopathology·2024
Same author

Ivacaftor for Chronic Obstructive Pulmonary Disease: Results from a Phase 2, Randomized Controlled Trial.

American journal of respiratory and critical care medicine·2024
Same author

Prime editing-mediated correction of the CFTR W1282X mutation in iPSCs and derived airway epithelial cells.

PloS one·2023
Same journal

The AP2α/PDHA1 signaling pathway promotes lung cancer progression by mediating aerobic glycolysis and inhibiting cuproptosis.

Experimental cell research·2026
Same journal

Distributed transcription activity in DLX proteins defies conventional mapping of a transcription activation domain.

Experimental cell research·2026
Same journal

Extracellular release of cytotoxic aggregates from HSV-1-replicating SH-SY5Y cells: involvement of alpha-synuclein and poly-ubiquitin conjugates.

Experimental cell research·2026
Same journal

MiR-1281 downregulates LMX1B to inhibit gastric cancer development.

Experimental cell research·2026
Same journal

Corrigendum to "Heat shock protein 90β stabilizes focal adhesion kinase and enhances cell migration and invasion in breast cancer cells" [Exp. Cell Res., 326, 1, 1 August 2014, Pages 78-89].

Experimental cell research·2026
Same journal

Neutrophilic differentiation of promyelocytic HL-60 cells is associated with increased expression of terminal galactose residues on the cell surface.

Experimental cell research·2026
See all related articles

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Ewing family tumors are driven by EWS/ets chimeric transcription factors.
  • The oncogenic activity of EWS/FLI is context-dependent.
  • Understanding cellular context is crucial for EWS/FLI function.

Purpose of the Study:

  • To investigate why EWS/FLI transformation is restricted to certain cellular contexts.
  • To identify cellular factors influencing EWS/FLI oncogenic activity.
  • To explore the role of downstream mediators like PDGF-C.

Main Methods:

  • Utilized immortalized fibroblast cell lines (Rat1, Yal7, NIH3T3).
  • Expressed EWS/FLI protein in resistant and sensitive cell lines.
  • Assessed transformation potential and downstream gene expression (e.g., PDGF-C).

Main Results:

  • Rat1 and Yal7 cells resist EWS/FLI transformation but can be transformed by PDGF-C.
  • EWS/FLI does not upregulate PDGF-C in resistant cell lines, unlike NIH3T3 cells.
  • Differential EWS/FLI target gene regulation observed across cell lines.

Conclusions:

  • PDGF-C is a key mediator of EWS/FLI-induced transformation.
  • Cellular background significantly influences EWS/FLI function and downstream effects.
  • Caution is advised when extrapolating oncogene function across different cellular environments.