Fluorescent complex of pyoverdin with aluminum
A del Olmo1, C Caramelo, C SanJose
1Departamento de Nutrición y Bromatologi;a III, Facultad de Veterinaria, Universidad Complutense de Madrid, Ciudad Universitaria, 28040, Madrid, Spain.
Journal of Inorganic Biochemistry
|October 22, 2003
Abstract:
When a pyoverdin (PV), (a siderophore) from Pseudomonas fluorescens, binds aluminum 1:1, its natural fluorescence almost doubles, whereas PV-Fe is non-fluorescent. Complex formation allows [Al] determination down to 1 mug/l. Fe(III) in the sample interferes with [Al] determination, but added after PV, improves the assay's performance. Ascorbic acid does not eliminate Fe(III) interference. PV-Al fluorescence could have analytical and toxicological applications.
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