Single-trial variability in early visual neuromagnetic responses: an explorative study based on the regional...

Cortical activity evoked by repeated identical sensory stimulation is extremely variable. The source of this variability is often assigned to "random ongoing background activity" which is considered to be irrelevant to the processing of the stimuli and can therefore be eliminated by ensemble averaging. In this work, we studied the single-trial variability in neuromagnetic responses elicited by circular checkerboard pattern stimuli with radii of 1.8 degrees, 3.7 degrees, and 4.5 degrees. For most of the MEG sensors over the occipital areas, the averaged signal showed a clear early (N70m) response following the stimulus onset and this response was modulated by the checkerboard size. A data-driven spatial filter was used to extract one of the many possible composite time courses of single-trial activity corresponding to the complex of N70m generators. Pattern analysis principles were then employed to analyze, classify, and handle the extracted temporal patterns. We explored whether these patterns correspond to distinct response modes, which could characterize the evoked response better than the averaged signal and over an extended range of latencies around N70m. A novel scheme for detecting and organizing the structure in single-trial recordings was utilized. This served as a basis for comparisons between runs with different checkerboard sizes and provided a causal interpretation of variability in terms of regional dynamics, including the relatively weak activation in primary visual cortex. At the level of single trial activity, the polymorphic response to a simple stimulus is generated by a coupling of polymodal areas and cooperative activity in striate and extrastriate areas. Our results suggest a state-dependent response with a wide range of characteristic time scales and indicate the ongoing activity as a marker of the responsiveness state.