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Genotyping patients with recent blood transfusions.
Michelle Ng Gong1, Yang Sai, Wei Zhou
1Pulmonary and Critical Care Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Epidemiology (Cambridge, Mass.)
|October 22, 2003
Summary
Microchimerism from blood transfusions rarely impacts genetic typing in intensive care units. High agreement between blood and buccal cell genotypes suggests reliable results, especially when DNA is collected soon after transfusion.
Area of Science:
- Genetics
- Critical Care Medicine
- Molecular Epidemiology
Background:
- Polymerase chain reaction amplification (PCR) is used for genotyping in intensive care units (ICUs).
- Donor leukocytes (microchimerism) from blood transfusions may interfere with recipient genotyping.
- Investigated potential interference of microchimerism on genotyping in ICU patients.
Purpose of the Study:
- To assess the impact of microchimerism from blood transfusions on genetic polymorphism genotyping in ICU patients.
- To compare genotyping results from blood and buccal cells in critically ill patients.
Main Methods:
- Collected blood and buccal cells from 145 ICU patients.
- Extracted DNA and performed genotyping for TNF-beta and surfactant protein-B gene polymorphisms.
- Utilized enzyme digestion and pyrosequencing for genotyping.
Main Results:
- High Kappa statistics (0.95-0.98) indicated strong agreement between blood and buccal cell genotypes.
- Analysis restricted to 107 transfused patients also showed high agreement (0.93-0.97).
- Microchimerism did not significantly affect genotyping accuracy.
Conclusions:
- Microchimerism from allogeneic transfusions has minimal impact on common polymorphism genotyping in critical care settings.
- Reliable genotyping results are achievable if DNA is collected within a day post-transfusion.
- Supports the validity of molecular epidemiology studies in transfused ICU populations.