Comparative study of the dental substrate used in shear bond strength tests

The purpose of this study was to compare shear bond strength values obtained in human enamel and dentin with the values obtained in bovine teeth using two adhesive systems with different actions. Forty human tooth half-crowns and forty bovine tooth crowns were flattened to a minimum plain area of 5 mm in diameter. The samples were divided in four groups of 20 specimens each: 1) human enamel; 2) bovine enamel; 3) human dentin; 4) bovine dentin. The samples of each group were divided in 2 subgroups of 10 samples each, according to the adhesive system used: 1) Scotchbond Multi-Purpose (SBMP); and 2) Clearfil Liner Bond 2V (CLB2V) applied according to the manufacturer's recommendations. Afterwards, restorations of Z100 composite with cylindrical shape (4 mm diameter x 5 mm height) were made using a metallic mold to submit the samples to shear bond testing on an Instron universal testing machine, at a crosshead speed of 0.5 mm/min. The data were submitted to ANOVA and Tukey's test (5%). In enamel, there was no statistical difference between bovine and human teeth for SBMP (7.36 MPa, human; 8.24 MPa, bovine), nor for CLB2V (10.01 MPa, human; 7.95, bovine). In dentin, SBMP showed a statistically lower mean on human dentin (7.01 MPa) than on bovine dentin (11.74 MPa). For CLB2V, there was no statistical difference between human (7.43 MPa) and bovine (9.27 MPa) substrates.