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Molecular identification of pathogenic and nonpathogenic strains of Vibrio harveyi using PCR and RAPD

G Hernández1, J Olmos

  • 1Molecular Microbiology Laboratory, Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Department of Marine Biotechnology, Ensenada, B.C. México.

Applied Microbiology and Biotechnology
|October 22, 2003
PubMed

Insights

Two Vibrio spp. strains (Z2 and Z3) from shrimp were pathogenic, causing 100% mortality. Molecular methods like PCR and RFLP distinguished virulent from avirulent Vibrio harveyi strains.

Area of Science:

  • Aquatic microbiology
  • Shrimp aquaculture
  • Bacterial pathogenicity

Background:

  • Vibrio species are significant pathogens in shrimp aquaculture.
  • Differentiating virulent from avirulent strains is crucial for disease management.

Purpose of the Study:

  • To assess the pathogenicity of environmental Vibrio isolates from shrimp.
  • To molecularly characterize and differentiate pathogenic and non-pathogenic Vibrio strains.

Main Methods:

  • Pathogenicity testing on juvenile shrimp.
  • Molecular characterization using Restriction Fragment Length Polymorphism (RFLP).
  • Polymerase Chain Reaction (PCR) with specific primers (e.g., luxN) and Random Amplified Polymorphic DNA (RAPD).

Main Results:

  • Two environmental isolates (strains Z2 and Z3) demonstrated 100% pathogenicity, causing significant shrimp mortality.
  • PCR using luxN primers confirmed all tested environmental strains as Vibrio harveyi.
  • RAPD successfully differentiated between virulent and avirulent Vibrio harveyi strains.

Conclusions:

  • Environmental Vibrio isolates can be highly pathogenic to shrimp.
  • Molecular techniques, including PCR and RFLP, are effective tools for identifying and distinguishing virulent Vibrio harveyi strains.
  • These methods aid in detecting and managing shrimp diseases caused by Vibrio.

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