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A preliminary linkage map of the tick, Ixodes scapularis
Amy J Ullmann1, Joseph Piesman, M C Dolan
1Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Rampart Road, Foothills Campus, Fort Collins, CO 80522, USA. aff1@cdc.gov
Abstract:
A linkage map of the Ixodes scapularis genome was constructed based upon segregation amongst 127 loci. These included 84 random amplified polymorphic DNA (RAPD) markers, 32 Sequence-Tagged RAPD (STAR) markers, 5 cDNAs, and 5 microsatellites in 232 F1 intercross progeny from a single, field-collected P1 female. A preliminary linkage map of 616 cM was generated across 14 linkage groups with one marker every 10.8 cM. Assuming a genome size of approximately 10(9) bp, the relationship of physical to genetic distance is approximately 300 kb/cM in the I. scapularis genome.