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Updated: Jul 19, 2026
12:04
Mouse Genome Engineering Using Designer Nucleases
Published on: April 2, 2014
Genomic organization of the mouse OSF-1 gene
1Pharma Research Laboratories, Hoechst Japan Limited, Saitama.
DNA and Cell Biology
|December 1, 1992
Summary
The mouse OSF-1 gene, crucial for bone and brain development, shares structural similarities with the midkine (MK) gene. This suggests OSF-1 and MK evolved from a common ancestral gene, indicating a related protein family.
Area of Science:
- Genetics
- Molecular Biology
- Developmental Biology
Background:
- The OSF-1 protein (also known as pleiotrophin) is a secreted protein found in bone and brain.
- It is implicated in brain development and osteogenesis.
Purpose of the Study:
- To isolate and sequence the mouse OSF-1 gene.
- To analyze its regulatory elements and compare its structure to related genes.
Main Methods:
- Genomic library screening and DNA sequencing.
- Bioinformatic analysis of 5'-flanking sequences.
- cDNA cloning and sequence analysis in osteoblastic cells.
Main Results:
- The mouse OSF-1 gene spans over 32 kb and contains at least 5 exons and 4 introns.
- Two candidate promoter regions were identified, one with thyroid hormone/retinoic acid-responsive elements and another with glucocorticoid-responsive elements.
- OSF-1 gene organization and exon-intron boundaries are similar to the mouse midkine (MK) gene.
Conclusions:
- OSF-1 and MK are likely derived from a common ancestral gene.
- OSF-1 and MK represent a family of structurally and functionally related proteins.
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