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Related Experiment Videos

Peroxynitrite decreases dopamine's vasoconstrictive activity.

Ko Takakura1, Wen Xiaohong, Kenji Takeuchi

  • 1*Department of Anesthesiology, Asahi University, Hozumi, Gifu; and †Department of Anesthesiology and Reanimatology, Fukui Medical University, Matsuoka, Japan.

Anesthesia and Analgesia
|October 23, 2003
PubMed
Summary
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Peroxynitrite oxidizes dopamine, reducing its vasoconstrictive ability. This finding explains dopamine

Area of Science:

  • Pharmacology
  • Physiology
  • Biochemistry

Background:

  • Nitric oxide (NO) and superoxide (O2(-1)) are key signaling molecules.
  • Increased NO and O2(-1) production occurs during septic shock.
  • Dopamine is used as a vasoconstrictor in critical care settings.

Purpose of the Study:

  • To investigate the vasoconstrictive activity of dopamine oxidized by peroxynitrite (ONOO(-1)).
  • To determine if ONOO(-1) affects dopamine's ability to constrict blood vessels.
  • To explore dopamine's potential limitations as a vasoconstrictor in conditions like septic shock.

Main Methods:

  • Isolated rat thoracic aorta strips were used for functional examinations.
  • Dopamine was treated with ONOO(-1) or SIN-1 (an ONOO(-1) producer).

Related Experiment Videos

  • Methylene blue, a guanylyl cyclase inhibitor, was used to block direct ONOO(-1) effects.

    • Main Results:

    • Dopamine induced concentration-dependent contractions.
    • Pretreatment with ONOO(-1) decreased dopamine's vasoconstrictive effect in a concentration-dependent manner.
    • Maximum contractions and EC50 values for dopamine were significantly reduced after ONOO(-1) pretreatment.

    Conclusions:

    • Peroxynitrite reacts with dopamine to form an oxidized derivative with reduced vasoconstrictive activity.
    • The decrease in vasoconstrictive activity was confirmed using SIN-1, an ONOO(-1) producer.
    • These findings suggest that elevated ONOO(-1) levels in septic shock may limit dopamine's effectiveness as a vasoconstrictor.