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Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
The Ras Gene02:38

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a superfamily...

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Related Experiment Video

Updated: Jul 11, 2026

Temporal Quantification of MAPK Induced Expression in Single Yeast Cells
07:59

Temporal Quantification of MAPK Induced Expression in Single Yeast Cells

Published on: October 5, 2013

Transcriptome profiling of a Saccharomyces cerevisiae mutant with a constitutively activated Ras/cAMP pathway.

D L Jones1, J Petty, D C Hoyle

  • 1Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology, Manchester M60 1QD, United Kingdom.

Physiological Genomics
|October 23, 2003
PubMed
Summary

Small gene expression changes are biologically significant, as shown by a statistical microarray analysis of a yeast mutant. This study reveals the transcriptome-wide cellular response to constitutive activation of the Ras/cAMP pathway.

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Published on: October 22, 2018

Area of Science:

  • Molecular Biology
  • Genomics
  • Yeast Genetics

Background:

  • Gene expression changes are often assessed using arbitrary thresholds, potentially overlooking subtle yet significant alterations.
  • The Ras/cAMP pathway plays a crucial role in cellular responses to various stimuli.
  • Understanding gene expression dynamics is key to deciphering cellular functions and responses.

Purpose of the Study:

  • To investigate the biological significance of small gene expression changes using a statistical approach in microarray analysis.
  • To perform a global transcriptome analysis of a Saccharomyces cerevisiae mutant with constitutive Ras/cAMP pathway activation.
  • To identify genes and pathways affected by altered Ras/cAMP signaling and their functional implications.

Main Methods:

  • Whole genome microarray analysis was performed on a pde2Delta mutant in Saccharomyces cerevisiae.
  • A purely statistical approach was applied to analyze gene expression data, moving beyond arbitrary thresholds.
  • Munich Information Centre for Protein Sequences (MIPS) functional categories were used to interpret gene expression patterns.

Main Results:

  • Approximately 11% of protein-encoding genes showed altered expression in the mutant.
  • Genes involved in ribosome biogenesis were upregulated, while those in cell rescue, defense, and aging were downregulated, suggesting impaired stress response.
  • Reduced expression in the unfolded protein response (UPR) pathway and alterations in cell wall genes were observed.
  • Several cAMP-responsive orphan genes were identified with novel or previously unknown functions, including roles in cell wall integrity.

Conclusions:

  • Small, statistically significant changes in gene expression hold biological relevance.
  • Constitutive activation of the Ras/cAMP pathway in yeast leads to broad transcriptome alterations, impacting stress response, metabolism, and cell wall integrity.
  • This study provides a comprehensive statistical transcriptome analysis of the cellular response to constitutive Ras/cAMP pathway activation, uncovering new gene functions.