Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Experimental bone marrow fat necrosis.

F Oberling, J P Cazenave

    Virchows Archiv. A, Pathological Anatomy and Histology
    |November 17, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Saponin injection causes bone marrow fat necrosis via microcirculation damage. This fat cell death may contribute to myelofibrosis and shows varied responses based on bone marrow location.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Adhesion and Transendothelial Migration of Human Monocytes: Role of Cytokine (IL3, GM-CSF) Activated Endothelial Cells.

    Hematology (Amsterdam, Netherlands)·2016
    Same author

    Are CD44 spliced variants involved in human breast cancer metastasis?

    Oncology reports·2011
    Same author

    Expression of CD44 spliced variants with both exons v9 and v10 in primary N(-)M(-) breast carcinomas correlates with metastasis.

    International journal of oncology·2011
    Same author

    Randomized, controlled, dose-range study of Ro 25-8315 given before and after a high-dose combination chemotherapy regimen in patients with metastatic or recurrent breast cancer patients.

    Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2002
    Same author

    Antithrombotic effects of (n-3) polyunsaturated fatty acids in rat models of arterial and venous thrombosis.

    Thrombosis research·1999
    Same author

    Polyethylenimine-mediated transfection of human monocytes with the IFN-gamma gene: an approach for cancer adoptive immunotherapy.

    Gene therapy·1999
    Same journal

    Primary carcinoid tumor of the ear.

    Virchows Archiv. A, Pathological anatomy and histology·1982
    Same journal

    Epithelial dysplasias in chronic pancreatitis.

    Virchows Archiv. A, Pathological anatomy and histology·1982
    Same journal

    Pathology of chronic vitamin E deficiency in fatal familial intrahepatic cholestasis (Byler disease).

    Virchows Archiv. A, Pathological anatomy and histology·1982
    Same journal

    An ultrastructural and morphometric study of bladder tumours (II).

    Virchows Archiv. A, Pathological anatomy and histology·1982
    Same journal

    Striae: morphological aspects of connective tissue.

    Virchows Archiv. A, Pathological anatomy and histology·1982
    Same journal

    Peanut lectin: a useful tool for detecting Hodgkin cells in paraffin sections.

    Virchows Archiv. A, Pathological anatomy and histology·1982
    See all related articles

    Area of Science:

    • Histopathology
    • Microcirculation
    • Bone Marrow Biology

    Background:

    • Saponin is known to affect cellular membranes.
    • Bone marrow adipose tissue plays a role in hematopoiesis and marrow microenvironment.
    • Ischemia is a critical factor in tissue damage and subsequent repair or pathological processes.

    Purpose of the Study:

    • To investigate the histopathological changes in rabbit bone marrow adipose tissue following saponin administration.
    • To elucidate the role of microcirculatory destruction in the development of steatonecrosis.
    • To explore the potential contribution of bone marrow fat necrosis to myelofibrosis pathogenesis.

    Main Methods:

    • Serial light and electron microscopy.
    • Intravenous injection of saponin in rabbits.

    Related Experiment Videos

  • Histopathological analysis of femur and sternum bone marrow.

    • Main Results:

    • Two distinct steatonecrosis patterns observed: giant-cell granulomas in femur and calcified foci in sternum.
    • Lesions originated from saponin-induced destruction of bone marrow microcirculation.
    • Necrosis of fat cells followed by calcium deposits in hematopoietic marrow versus giant-cell reaction in fatty marrow.

    Conclusions:

    • Bone marrow fat cell necrosis, induced by microcirculatory ischemia, can contribute to myelofibrosis.
    • Adipose tissue exhibits differential responses to ischemia based on anatomical location within the bone marrow.