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Updated: Aug 14, 2026

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
Published on: July 21, 2014
Identifying global regulators in transcriptional regulatory networks in bacteria
Agustino Martínez-Antonio1, Julio Collado-Vides
1Program of Computational Genomics, CIFN, Universidad Nacional Autónoma de México A. P. 565-A Cuernavaca, 62100, Morelos, Mexico. agustino@cifn.unam.mx
Abstract:
The machinery for cells to take decisions, when environmental conditions change, includes protein-DNA interactions defined by transcriptional factors and their targets around promoters. Properties of global regulators are revised attempting to reach diagnostic explicit criteria for their definition and eventual future computational identification. These include among others, the number of regulated genes, the number and type of co-regulators, the different sigma-classes of promoters and the number of transcriptional factors they regulate, the size of the evolutionary family they belong to, and the variety of conditions where they exert their control. As a consequence, global versus local regulation can be identified, as shown for Escherichia coli and eventually in other genomes.
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