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Related Experiment Videos

MIR phasing using merohedrally twinned crystals.

Anke C Terwisscha van Scheltinga1, Karin Valegård, Janos Hajdu

  • 1Department of Molecular Biology, Swedish University of Agricultural Sciences, Box 590, S-751 24 Uppsala, Sweden. anke@xray.bmc.uu.se

Acta Crystallographica. Section D, Biological Crystallography
|October 24, 2003
PubMed
Summary

Merohedral twinning complicates macromolecular structure determination. Strategies to manage twin fraction and detwin data are crucial for successful crystal structure analysis.

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Area of Science:

  • Crystallography
  • Structural Biology
  • Biophysics

Background:

  • Merohedral twinning is a common crystal-growth disorder.
  • It significantly impedes macromolecular crystal structure determination using methods like isomorphous replacement.
  • Overcoming twinning is essential for advancing structural biology.

Purpose of the Study:

  • To discuss strategies for addressing merohedral twinning in macromolecular crystallography.
  • To review methods for determining and mitigating the effects of twinning on crystal structure determination.

Main Methods:

  • Analysis of strategies employed in previously solved twinned crystal structures.
  • Methods for determining the extent of twinning and the twin fraction.
  • Techniques for detwinning crystallographic data.

Related Experiment Videos

  • Heavy-atom refinement statistics for twin fraction accuracy.

    • Main Results:

    • Accurate twin fraction determination is possible via heavy-atom refinement statistics.
    • Twin fraction variations between datasets can be minimized by artificial increases or crystal screening.
    • Phase accuracy is only slightly influenced by precise twin fraction determination.

    Conclusions:

    • Managing twin fraction is critical for successful macromolecular structure determination.
    • Strategies include controlling twin fraction variation and employing detwinning methods.
    • Further research into optimizing twinning correction techniques is warranted.