Analysis of peptides of human seminal plasma by mass spectrometry

A procedure is described to isolate peptides from complex biological fluids (e.g. seminal plasma) in pure form within a short time. The sequence of the isolated peptides was determined by liquid secondary ion mass spectrometry in combination with enzymatic cleavage reactions. The method was used for the structure elucidation of a peptide of molecular weight 2766 u with an N-terminal pyroglutamine residue.