Related Experiment Video
Updated: Aug 30, 2026
Quantification of Autoreactive Antibodies in Mice upon Experimental Autoimmune Encephalomyelitis
Published on: December 1, 2023
[Study on autoimmune thrombocytopenic purpura related antibody]
1Department of Hematology, Qilu Hospital, Shandong University, Jinan 250012, China.
Objective:
To investigate a specific and sensitive assay for the diagnosis of autoimmune thrombocytopenic purpura (AITP).
Methods:
Glycoprotein specific autoantibodies in platelet eluate and plasma were detected by a modified monoclonal antibody immobilization of platelet antigens assay (MAIPA).
Results:
The overall positive rate of specific autoantibodies against platelet GPIIb/IIIa and GPIb/IX in plasma was 38.89% and in eluated platelet membrane was 68.52%. The difference between them was significant (corrected chi(2) = 19.39, P < 0.005). The proportion of positive MAIPA results between primary AITP and secondary AITP was not significantly different. There was a significant inverse correlation between antibody level and platelet count.
Conclusion:
Detection of eluated GP-specific autoantibodies by MAIPA is highly specific and much more sensitive as compared with the measurement of their plasma counterparts in the diagnosing and therapeutic monitoring of AITP.
