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Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
Published on: March 8, 2012
[Study on the correlation between the different papillomavirus type and telomerase in cervical cancer]
Wen Lv1, Guang-Mei Zhang, Li-Hua Sui
1Department of Gynecology, the Third Affiliated Hospital Harbin Medical University, Harbin 150040, China.
Objective:
To define a correlation between different human papillomavirus (HPV) types and telomerase activity in cervical cancer.
Methods:
Telomerase activity was detected by TRAP-PCR, and different HPV type was determined by PCR in 83 cervical cancer, 47 cervical intraepithelial neoplasia (CIN) and 10 normal cervix cases.
Results:
With regard to positive rates of telomerase and HPV 16/18: the results were cervical cancer > CIN > normal cervix, CIN III > CIN I, II; with regard to HPV 6/11 positive rate: the results showed CIN I, II > CIN III. Positive rates of telomerase cervical cancer and HPV were bearing on grading and staging, but they did not correlate with histologic subtypes. Positive rate of HPV 6/11 had nothing to do with grading, staging and histologic patterns. On expression strength of telomerase and HPV 16/18: the results showed cervical cancer > CIN, CIN III > CIN I, II. Regard to HPV 6/11'expression strength: the results showed CIN I, II > CIN III, CIN > cervical carcinoma.
Conclusion:
HPV 16/18 infection seemed to have played an important role in carcinogenesis of cervical lesions by activation of telomerase.
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