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Transcriptional activation of mouse mast cell protease-9 by microphthalmia-associated transcription factor

Masaru Murakami1, Teruo Ikeda, Kenji Ogawa

  • 1Laboratory of Molecular Biology, Azabu University School of Veterinary Medicine, Sagamihara, Japan.

Biochemical and Biophysical Research Communications
|October 25, 2003
PubMed

Insights

Microphthalmia-associated transcription factor (MITF) positively regulates mouse mast cell protease-9 (mMCP-9) transcription. This regulation is crucial for mMCP-9

Area of Science:

  • Molecular Biology
  • Immunology
  • Developmental Biology

Background:

  • Mouse mast cell protease-9 (mMCP-9) is involved in helminth-induced jejunal inflammation and uterine tissue remodeling during pregnancy.
  • Transcriptional regulation of mMCP-9 is critical for understanding its role in these physiological and pathological processes.

Purpose of the Study:

  • To elucidate the transcriptional regulation of mMCP-9.
  • To identify the specific transcription factors and DNA sequences involved in mMCP-9 gene expression.

Main Methods:

  • Analysis of the mMCP-9 promoter region.
  • Electrophoretic mobility shift assays (EMSAs) to study transcription factor binding.
  • Reporter gene assays to assess transcriptional activity.

Main Results:

  • Microphthalmia-associated transcription factor (MITF) was identified as a positive regulator of mMCP-9 transcription.
  • The mMCP-9 promoter region from nucleotide -183 to -177 (CATCATG) was found to be a key binding site for MITF-M.
  • Other MITF isoforms and TFE3 also activated mMCP-9 transcription via this E-box-proximal sequence.

Conclusions:

  • MITF and TFE3 play significant roles in the transcriptional activation of mMCP-9.
  • The identified promoter region and its interaction with MITF are critical for mMCP-9 gene expression in relevant biological contexts.

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