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Updated: Aug 14, 2026
13:20
Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells
Published on: July 30, 2010
[Structural changes of chromatin during proto-oncogene activation by cycloheximide: dose-effect]
Biokhimiia (Moscow, Russia)
|October 1, 1992
Summary
Cellular proto-oncogenes c-myc and c-fos expression in rat liver is triggered by inhibiting nuclear protein synthesis. This process involves chromatin structural changes and may involve topoisomerase II.
Area of Science:
- Molecular Biology
- Cellular Biology
- Oncology
Context:
- Investigates the expression of cellular proto-oncogenes c-myc and c-fos in rat liver.
- Examines the relationship between protein synthesis rates and proto-oncogene activation.
- Utilizes cycloheximide to modulate protein synthesis.
Purpose:
- To determine the effect of inhibited protein synthesis on proto-oncogene expression.
- To elucidate the molecular mechanisms underlying proto-oncogene activation.
- To explore the role of chromatin structure in gene regulation.
Summary:
- Proto-oncogene activation (c-myc, c-fos) in rat liver occurs at 50% inhibition of nuclear protein synthesis.
- Inhibition of protein synthesis induces chromatin structural rearrangements.
- These rearrangements include decreased Ca2+, Mg(2+)-DNAase activity and increased chromatin condensation.
Impact:
- Suggests a role for topoisomerase II in chromatin remodeling during proto-oncogene activation.
- Provides insights into the regulation of key oncogenes.
- Contributes to understanding the early events in cellular transformation.
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