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Related Experiment Video

Updated: Jul 8, 2026

Performing Vaginal Lavage, Crystal Violet Staining, and Vaginal Cytological Evaluation for Mouse Estrous Cycle Staging Identification
09:01

Performing Vaginal Lavage, Crystal Violet Staining, and Vaginal Cytological Evaluation for Mouse Estrous Cycle Staging Identification

Published on: September 15, 2012

LacZ expression in Fut2-LacZ reporter mice reveals estrogen-regulated endocervical glandular expression during

Steven E Domino1, Elizabeth A Hurd

  • 1Department of Obstetrics and Gynecology, Cellular and Molecular Biology Program, 6428 Medical Science I Box 0617, University of Michigan Medical Center, Ann Arbor, MI 48109-0617, USA. sedomino@med.umich.edu

Glycobiology
|October 25, 2003
PubMed
Summary
This summary is machine-generated.

The secretor gene (FUT2) is regulated by hormones in female reproductive tissues. FUT2 expression in the cervix and uterus changes during the estrous cycle and pregnancy, suggesting a role in barrier function.

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Last Updated: Jul 8, 2026

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09:01

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Exploring Independent Effects of Follicle-Stimulating Hormone In Vivo in a Mouse Model
05:32

Exploring Independent Effects of Follicle-Stimulating Hormone In Vivo in a Mouse Model

Published on: August 11, 2023

Area of Science:

  • Reproductive biology
  • Glycobiology
  • Gene regulation

Background:

  • The secretor gene (FUT2) encodes an alpha(1,2)fucosyltransferase involved in creating fucosylated glycans.
  • While proposed for embryo adhesion, FUT2's function in female reproduction is unclear, as Fut2-null mice show normal fertility.

Purpose of the Study:

  • To investigate the cell-specific expression and hormonal regulation of Fut2 in the female reproductive tract.
  • To explore alternative hypotheses for FUT2 function beyond embryo adhesion.

Main Methods:

  • Utilized Fut2-LacZ reporter mice to track Fut2 expression.
  • Examined expression patterns during the estrous cycle, hormone replacement, and pregnancy.
  • Performed Northern blot analysis to quantify Fut2 mRNA levels in wild-type mice.

Main Results:

  • Fut2 expression was prominent in the endocervix glandular epithelium during estrus and pregnancy.
  • Estradiol treatment increased Fut2 expression in the endocervix and uterus but not stomach or colon.
  • Uterine Fut2 mRNA levels varied eightfold during the estrous cycle, with significant elevations during estrus and pregnancy.

Conclusions:

  • Demonstrated tissue-specific hormonal regulation of a glycosyltransferase gene (FUT2) in a reporter mouse model.
  • Endocervical FUT2 expression during estrus and pregnancy may influence cervical mucus properties, potentially protecting against microbial infection.