Influence of reagent composition on atypical pseudocholinesterase activity measurement: comparison of a manual and an automated method and implications for routine
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N B Krull1, J Kropf, A M Gressner
1Dept. of Clinical Chemistry, Philipps-University, Marburg, Germany.
Abstract:
In determining activities of atypical pseudocholinesterase using a Hitachi 737 and a Cobas Bio analyser, respectively, we repeatedly found discrepancies between the enzyme activities measured by both instruments which were presumably due to differences in the reagent composition (Hitachi 737: Boehringer Mannheim Automated Analysis for BM/Hitachi Systems; Cobas Bio: Boehringer Mannheim Monotest). We consider the present results as a strong hint for manufacturers towards generally declaring all components of the chemical systems they produce and as a warning for users when interchanging apparently identically composed sets of reagents.