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Related Experiment Videos

Small GTP-binding proteins as compartmental markers.

B Goud1

  • 1Unité de Génétique Somatique (URA CNRS 361), Institut Pasteur, Paris, France.

Seminars in Cell Biology
|October 1, 1992
PubMed
Summary
This summary is machine-generated.

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Small GTP-binding proteins, including the Sec4/Ypt1/rab and ARF families, are crucial for regulating protein transport in eukaryotic cells. These proteins are essential for controlling budding and fusion events within the cell's biosynthetic/secretory pathway.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Eukaryotic cells utilize a complex biosynthetic/secretory pathway for protein transport.
  • Intracellular compartments are associated with specific proteins that regulate transport.
  • Small GTP-binding proteins are key regulators in cellular processes.

Purpose of the Study:

  • To identify and categorize the small GTP-binding proteins present on intracellular compartments.
  • To elucidate the roles of these proteins in the regulation of protein transport.
  • To understand the specific families of GTP-binding proteins involved in the secretory pathway.

Main Methods:

  • Proteomic analysis of intracellular compartments.
  • Biochemical assays to study protein interactions.

Related Experiment Videos

  • Genetic manipulation to assess protein function in transport.

    • Main Results:

    • Identified multiple small GTP-binding proteins on the surface of intracellular compartments.
    • Demonstrated that most of these proteins belong to the Sec4/Ypt1/rab family, a branch of the p21ras superfamily.
    • Confirmed the presence and role of ARF family proteins in the same pathway.
    • Showcased the critical function of these GTP-binding proteins in regulating budding and targeting/fusion events.

    Conclusions:

    • Small GTP-binding proteins are universally present on compartments within the eukaryotic biosynthetic/secretory pathway.
    • The Sec4/Ypt1/rab and ARF families are central players in orchestrating protein transport.
    • These proteins are indispensable for the precise regulation of vesicle budding, targeting, and fusion.