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Related Experiment Videos

Tissue viability measurement by in situ fluorometry.

C S Orr1, S C Arthurs

  • 1Biomedical Engineering Program, University of Texas Southwestern Medical Center, Dallas 75235-9031.

ASAIO Journal (American Society for Artificial Internal Organs : 1992)
|July 1, 1992
PubMed
Summary

A new laser-fiber optic sensor enables real-time monitoring of nicotinamide adenine dinucleotide (NADH) in tissues. This innovative system shows promise for detecting ischemia and hypoxia in clinical settings.

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Area of Science:

  • Biomedical Engineering
  • Optical Sensing
  • Biochemistry

Background:

  • Nicotinamide adenine dinucleotide (NADH) is a critical biomarker for cellular metabolism.
  • Monitoring NADH levels in situ is essential for diagnosing conditions like ischemia and hypoxia.
  • Existing methods for NADH detection often lack the sensitivity or real-time capabilities required for clinical applications.

Purpose of the Study:

  • To develop and evaluate a prototype laser-fiber optic sensor for in situ monitoring of NADH.
  • To assess the sensor's feasibility for continuous monitoring of ischemia and hypoxia indicators.
  • To introduce a novel dual-beam reflection method for compensating blood volume artifacts.

Main Methods:

  • Utilized a compact neodymium-yttrium-aluminum-garnet (Nd:YAG) laser with harmonic generators.
  • Employed a fiber optic network for light distribution to and from target tissue.
  • Immobilized lactate dehydrogenase on the fiber tip to create a lactate sensor.
  • Implemented a dual-beam reflection approach for artifact compensation.

Main Results:

  • Achieved detection sensitivity for free NADH in the micromolar range.
  • Demonstrated the capability for in situ monitoring of NADH.
  • Successfully tested a dual-beam reflection method for blood volume artifact compensation.
  • The system configuration proved feasible for continuous monitoring.

Conclusions:

  • The developed laser-fiber optic sensor is a viable tool for in situ NADH monitoring.
  • The system shows potential for continuous clinical assessment of ischemia and hypoxia.
  • The dual-beam reflection technique effectively compensates for blood volume artifacts, enhancing measurement accuracy.

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