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Related Experiment Videos

Heparin immobilization by surface amplification.

A Z Piao1, H A Jacobs, K D Park

  • 1Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City 84108.

ASAIO Journal (American Society for Artificial Internal Organs : 1992)
|July 1, 1992
PubMed
Summary

Researchers developed a novel method to immobilize heparin (HEP) on polymer surfaces, significantly increasing its amount and bioactivity. This technique enhances heparin

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Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Surface Modification

Background:

  • Heparin immobilization on polymer surfaces is crucial for biomedical applications, particularly in preventing thrombosis.
  • Existing methods often result in limited heparin loading and reduced bioactivity.
  • Improving the efficiency and efficacy of heparinized surfaces remains a significant challenge.

Purpose of the Study:

  • To develop an advanced method for enhancing heparin (HEP) immobilization on polyurethane-urea (PU) surfaces.
  • To increase the quantity of immobilized heparin and improve its biological activity.
  • To create more effective heparin-coated materials for biomedical use.

Main Methods:

  • Polyurethane-urea (PU) coated glass beads were modified using diisocyanates and surface grafting of polyfunctional polymers (PFP).
  • Isocyanate groups were amplified, followed by coupling of polyethylene oxide (PEO) and subsequent heparin immobilization.
  • Surface quantification of isocyanate groups and heparin content was performed throughout the process.

Main Results:

  • Grafted surfaces exhibited a four to eightfold increase in isocyanate group concentration.
  • Immobilized heparin content was twofold higher compared to direct immobilization on PU surfaces.
  • Heparin bioactivity, assessed by activated partial thromboplastin time, thrombin times, and factor Xa, was significantly enhanced.

Conclusions:

  • The developed method effectively increases heparin loading and bioactivity on polymer surfaces.
  • The PFP-PEO-mediated immobilization strategy offers a superior approach for heparinized biomaterials.
  • This technique holds promise for developing advanced anticoagulant surfaces with improved performance.

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