Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Insertional mutagenesis in zebrafish.

Adam Amsterdam1

  • 1Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. aha@mit.edu

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|October 28, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Library transgenesis in zebrafish through delayed site-specific mosaic integration for in vivo pooled screening of transgenes.

bioRxiv : the preprint server for biology·2026
Same author

MITF deficiency and oncogenic GNAQ each promote proliferation programs in zebrafish melanocyte lineage cells.

Pigment cell & melanoma research·2022
Same author

MITF deficiency accelerates GNAQ-driven uveal melanoma.

Proceedings of the National Academy of Sciences of the United States of America·2022
Same author

Uveal melanoma driver mutations in GNAQ/11 yield numerous changes in melanocyte biology.

Pigment cell & melanoma research·2018
Same author

Talin1 is required for cardiac Z-disk stabilization and endothelial integrity in zebrafish.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2015
Same author

Variations in dysfunction of sister chromatid cohesion in esco2 mutant zebrafish reflect the phenotypic diversity of Roberts syndrome.

Disease models & mechanisms·2015
Same journal

Multi-stage transcriptome analysis reveals genetic orchestration of rat testis development.

Developmental dynamics : an official publication of the American Association of Anatomists·2026
Same journal

Three-dimensional observation of the muscle-tendon integration process in mouse embryos.

Developmental dynamics : an official publication of the American Association of Anatomists·2026
Same journal

Goofy/123Cre lineage tracing differentiates olfactory and vomeronasal neurons from GnRH-1 and terminal nerve neurons during neuronal migration and reveals additional olfactory placode-derived cells in the brain.

Developmental dynamics : an official publication of the American Association of Anatomists·2026
Same journal

Prenatal sexual dimorphism in human pelvic tilt at the onset of fetal ossification.

Developmental dynamics : an official publication of the American Association of Anatomists·2026
Same journal

Meet the editorial team. An interview with Ralph Marcucio, Assistant Editor, University of California San Francisco, United States.

Developmental dynamics : an official publication of the American Association of Anatomists·2026
Same journal

Editorial highlights.

Developmental dynamics : an official publication of the American Association of Anatomists·2026
See all related articles

Retrovirus-mediated insertional mutagenesis in zebrafish efficiently identifies essential genes for development and survival. This review covers the methods and findings of this powerful genetic screening approach.

Area of Science:

  • Genetics
  • Developmental Biology
  • Molecular Biology

Background:

  • Insertional mutagenesis uses DNA integration to identify essential genes.
  • Retrovirus-mediated insertional mutagenesis is a key tool in zebrafish research.
  • Understanding gene function is crucial for developmental biology.

Purpose of the Study:

  • To review the methodology of retrovirus-mediated insertional mutagenesis in zebrafish.
  • To summarize the findings from insertional mutagenesis screens to date.
  • To explore future applications of insertional mutagenesis in genetic screens.

Main Methods:

  • Utilizing retrovirus integration as a mutagenic strategy.
  • Employing zebrafish as a model organism for genetic screens.

Related Experiment Videos

  • Facilitating gene cloning through DNA integration.

    • Main Results:

    • Identification of hundreds of genes essential for zebrafish embryonic development.
    • Discovery of genes critical for cell viability and growth.
    • Revealing the diversity of gene products involved in vertebrate development.

    Conclusions:

    • Retrovirus-mediated insertional mutagenesis is a highly effective method for gene discovery in zebrafish.
    • This approach has significantly advanced our understanding of developmental processes.
    • Further applications in genetic screening hold great potential for future research.