Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Validation of cDNA microarray technology].

Yao Luo1, Hong Xu, Yao Li

  • 1State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, China. yao_luo@hotmail.com

Yi Chuan Xue Bao = Acta Genetica Sinica
|October 29, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Magnesium sulfate provides neuroprotection in lipopolysaccharide-activated primary microglia by inhibiting NF-κB pathway.

The Journal of surgical research·2013
Same author

Loss of nerve fibers in the oviduct isthmus in women with hydrosalpinx.

Acta histochemica·2013
Same author

[Effect of P2X7 receptor on inflammatory diseases and its mechanism].

Sheng li xue bao : [Acta physiologica Sinica]·2013
Same author

Analysis of accuracy in pointing with redundant hand-held tools: a geometric approach to the uncontrolled manifold method.

PLoS computational biology·2013
Same author

In vitro cytotoxicity of CdSe/ZnS quantum dots with different surface coatings to human keratinocytes HaCaT cells.

Journal of environmental sciences (China)·2013
Same author

Diminished appetite predicts mortality of Chinese peritoneal dialysis patients.

Biological research for nursing·2013

This study validates cDNA microarray technology for measuring mRNA expression. Results demonstrate high accuracy and reproducibility, with most experimental errors reducible through replicate experiments.

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • cDNA microarray technology enables global measurement of mRNA expression levels.
  • Assessing the reliability and reproducibility of microarray data is crucial for accurate biological insights.

Purpose of the Study:

  • To verify the reproducibility and accuracy of cDNA microarray technique.
  • To investigate the influence of experimental factors on data quality.
  • To introduce a new parameter, Consistence Rate (CR), for evaluating reproducibility.

Main Methods:

  • Utilized self-comparison and differential expression experiments.
  • Analyzed data reliability using correlation coefficient (R), coefficient of variation (CV), and false positive rate (FPR).
  • Investigated factors including spotted DNA/mRNA/total RNA concentration, slide batches, and labeling processes.

Related Experiment Videos

Main Results:

  • Achieved correlation coefficients generally above 0.9.
  • Reported coefficient of variation around 15% and false positive rates below 3%.
  • Demonstrated that replicate experiments can significantly reduce experimental errors.

Conclusions:

  • cDNA microarray technology provides accurate and reproducible gene expression data.
  • Consistence Rate (CR) offers advantages over existing parameters for assessing reproducibility.
  • Optimizing experimental conditions and employing replicate experiments enhance data reliability.