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High-throughput Yeast Plasmid Overexpression Screen
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Direct retransformation of yeast with plasmid DNA isolated from single yeast colonies using rolling circle

Xiaodong Ding1, Anita K Snyder, Regina Shaw

  • 1University of Florida, Gainesville, FL, USA.

Biotechniques
|October 29, 2003
PubMed

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Transformation01:26

Transformation

Microbial communities are dynamic environments where cell lysis releases free DNA into the surroundings. Other cells can take up this extracellular DNA through a process known as transformation.When a cell incorporates this foreign DNA into its genome, resulting in genetic modification, the process is known as transformation. Cells capable of this process are termed competent. Competence can be natural, as observed in certain bacteria and archaea, or artificially induced in the...